Isolation of an N-acetyl-D-glucosamine specific lectin from the rhizomes of Arundo donax with antiproliferative activity |
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Authors: | Kaur Amandeep Singh Jatinder Kamboj Sukhdev Singh Sexana A K Pandita Renu Moti Shamnugavel M |
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Institution: | Department of Molecular Biology & Biochemistry, Guru Nanak Dev University, Amritsar 143 005, Punjab, India. |
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Abstract: | A lectin with antiproliferative activity towards human cancer cell lines and mitogenic towards human peripheral blood mononuclear cells was purified from the rhizomes of Arundo donax (Linn.) by affinity chromatography on N-acetyl-d-glucosamine linked to epoxy-activated sepharose-6B. The pure preparation apparently yielded a single band of approximately 15 kDa on SDS-PAGE, pH 8.3, under both reducing and non-reducing conditions. The molecular mass of native lectin was 32 kDa as determined by gel filtration chromatography. This showed the lectin to be a dimer, with subunits not held together by disulphide linkages. The A. donax lectin (ADL) agglutinated rabbit erythrocytes and the agglutination was inhibited by N-acetyl-d-glucosamine and its di- and trimer. The lectin was thermostable upto 55 degrees C and showed optimum activity in the range of pH 7.0-9.0 and comprised of 2.1% carbohydrate content. |
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Keywords: | d-glucosamine" target="_blank">N-acetyl-d-glucosamine Antiproliferative Arundo donax Lectin Mitogen |
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