Differential effects of tumor necrosis factor on the growth and differentiation of neuroblastoma and glioma cells |
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| Authors: | M A Mu?oz-Fernández R Armas-Portela J Díaz-Nido J L Alonso M Fresno J Avila |
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| Affiliation: | Centro de Biología Molecular (CSIC-UAM), Universidad Autónoma, Madrid, Spain. |
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| Abstract: | We have studied the effect of tumor necrosis factor (TNF-alpha) on transformed neural and glial-derived cell lines. TNF-alpha at physiological doses was able to arrest the growth and inhibit DNA synthesis of N103 neuroblastoma cells. This phenomenon was accompanied by a morphological cell differentiation characterized by the outgrowth of neurites. By contrast, TNF-alpha induced an increase in the growth rate of C6 glioma cells and upon cytokine addition a higher number of C6 cells were found in the S + G2 phase of the cell cycle. C6 cells did not show morphological changes under this treatment. Analogous results were obtained with IFN-gamma. These neurotrophic and mitogenic effects of TNF-alpha suggest a putative role of this cytokine in the regeneration of brain tissue upon brain injury. |
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