In vitro proliferation of shoots and regeneration of cotton |
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Authors: | Gupta Shiv K. Srivastava Alok K. Singh Pradhyumna K. Tuli Rakesh |
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Affiliation: | (1) National Botanical Research Institute, Rana Pratap Marg, Lucknow-, 226 001, India |
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Abstract: | Shoot proliferation from different explants of several Indian cultivars of cotton was studied in culture. Cotyledonary nodes taken along with the shoot apex of seedlings produced multiple shoots on modified MS nutrient agar supplemented with cytokinins. 6-Benzyladenine was most effective in inducing growth of multiple shoots. Explants of several genotypes formed organogenic masses that differentiated to secondary shoots on repeated subculture. The isolated shoots were rooted on basal medium supplemented with naphthaleneacetic acid and were transferred to soil after acclimatization. This revised version was published online in June 2006 with corrections to the Cover Date. |
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Keywords: | apical meristem cotyledonary node Gossypium sp micropropogation multiple shoots |
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