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The Role of Anaerobic Respiration in Germinating Muskmelon Seeds: II. EFFECT OF ANOXIA TREATMENT AND ALCOHOL DEHYDROGENASE ACTIVITY
Authors:PESIS  EDNA; NG  TIMOTHY J
Abstract:Exposing muskmelon (Cucumis melo L.) cultivar seedlots to N2atmosphere created totally anaerobic conditions which stimulatedethanol production and accumulation in both high and low vigourseeds. However, accumulation of ethanol was consistently higherin the low vigour seeds than in the high vigour ones. In addition,CO2 production under N2 and in air suggests the presence ofan apparent ‘Pasteur effect’ in the low vigour seedsbut not in the high vigour seeds. Acetaldehyde production underN2 was very low and did not seem to be associated with seedvigour, probably because of its nature as an intermediate inethanol production. The fast shift toward ethanol may be dueto the fact that alcohol dehydrogenase, the enzyme which catalyzesthe conversion of acetaldehyde to ethanol, exists in sufficientamounts in the imbibing seeds so that it is not a limiting factorin the conversion to ethanol. Alcohol dehydrogenase activitydid not appear to be related to seed vigour. Key words: Cucumis melo L., Anaerobic respiration, Germination, Seeds.
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