叶绿素缺失和异养生长对盐藻Synechocysitis Sp PCC6803藻胆蛋白含量影响

通过遮黑培养缺失frxC基因的蓝藻Synechocystis sp.PCC 6803突变工程株，获得了叶绿素缺失的藻细胞，吸收光谱测定及数学计算表明，藻细胞中叶绿素缺失后藻胆蛋白含量增加，藻蓝蛋白和别藻蓝蛋白含量分别为相同条件下野生株对照组的4倍和6倍。野生株遮黑培养时，细胞进行异养生长, 藻胆蛋白含量下降，藻蓝蛋白和别藻蓝蛋白含量分别为光照培养条件下自养生长的野生株细胞的34.5％和25.3％。另外，缺失apcE基因的突变工程株细胞的藻胆蛋白含量也少于对照野生株，表明apcE基础因的编码蛋白Lcm与藻胆蛋白的含量相关。

The Effects of Deletion of Chlorophyll and Heterotrophy on Phycobiliprotein Content of Cyanobacterium synechocystis sp. PCC6803

Under dark culture conditions, chlorophyll disappeared in the mutant cells of Cyanobacterium Synechocystis sp. PCC 6803 resulting from deletion of frxC gene. Absorption spectra and calculation indicated that the phycobiliprotein content increased in the chlorophyll - less algal cells. The contents of phycocyanin and allophycocyanin in the chlorophyll- less algal cells were four and six times respectively as much as those in wild- type cells under the same conditions. When wild-type cells grew heterotrophically in dark, the phycobiliprotein content decreased. The contents of phycocyanin and allophycocanin in the heterotrophic cells were 34.5 % and 25.3 % of those in the autotrophic cells grown in light, respectively. In addition, the phycobiliprotem content in the mutant cells with deletion of apcE gene was lower than that of wild - type cells, showing that the protein Lcm encoded by apcE gene was related to the phycobiliprotein content in algal cells.