The DNA sequence on bacteriophage G4 recognized by the Escherichia coli B restriction enzyme. |
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Authors: | J A Lautenberger M H Edgell C A Hutchison G N Godson |
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Affiliation: | Department of Bacteriology, School of Medicine University of North Carolina Chapel Hill, N.C. 27514, U.S.A.;Radiobiology Laboratories, Yale University School of Medicine 333 Cedar Street, New Haven, Conn. 06510, U.S.A. |
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Abstract: | Bacteriophage G4 possesses a single EcoB site located in the overlap between restriction fragments HinfI-12 and HaeIII-6. The sequence 5′-T-G-A … 8N … T-G-C-T occurs once in this segment and nowhere else in the DNA sequence of G4. Four independent G4 mutants that were not restricted by Escherichia coli B possessed the sequence 5′-T-G-A … 8N … T-G-C-C. The common sequence shared by the previously mapped EcoB sites on φXsB1, simian virus 40, f1, and fd DNAs is 5′-T-G-A … 8N … T-G-C-T … 9N … T. However, the sequence in the region of the G4 EcoB site contains an A instead of the final T conserved in these other examples. When the G4 EcoB site is aligned with the other EcoB sites, there are no conserved residues within 50 bases of the common sequence, 5′-T-G-A … 8N … T-G-C-T, except for those seven residues. The analysis of the EcoB site on G4 provides further evidence that only those seven bases are recognized by the E. coli B restriction enzyme. |
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