Upregulated mRNA expression of desaturase and elongase,two enzymes involved in highly unsaturated fatty acids biosynthesis pathways during follicle maturation in zebrafish |
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Authors: | Sairatul D Ishak Sze-Huey Tan Hou-Keat Khong Annette Jaya-Ram Yee-Ling Enyu Meng-Kiat Kuah Alexander Chong Shu-Chien |
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Affiliation: | (1) School of Biological Sciences, Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia;(2) Malaysian Institute of Pharmaceuticals and Nutraceuticals, Level 1, Universiti Sains Malaysia, J05 Building Science Complex, 11800 Minden, Penang, Malaysia |
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Abstract: | Background Although unsaturated fatty acids such as eicosapentaenoic acid (EPA, C20:5n-3), docosahexaenoic acid (DHA, C22:6n-3) and arachidonic acid (ARA, C20:4n-6), collectively known as the highly unsaturated fatty acids (HUFA), play pivotal roles in vertebrate reproduction, very little is known about their synthesis in the ovary. The zebrafish (Danio rerio) display capability to synthesize all three HUFA via pathways involving desaturation and elongation of two precursors, the linoleic acid (LA, C18:2n-6) and linolenic acid (LNA, C18:3n-3). As a prerequisite to gain full understanding on the importance and regulation of ovarian HUFA synthesis, we described here the mRNA expression pattern of two enzymes; desaturase (fadsd6) and elongase (elovl5), involved in HUFA biosynthesis pathway, in different zebrafish ovarian follicle stages. Concurrently, the fatty acid profile of each follicle stage was also analyzed. |
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