|
|||||
|
|
Some factors affecting successful vitrification of mouse blastocysts |
|
| Authors: | Van Der Zwalmen P Gaurois B Ectors F J Touati K Massip A Ectors F |
| Institution: | 1 Department of Animal Reproduction Faculty of Veterinary Medicine 45, rue des vétérinaires B 1070, Brussels, Belgium 2 Sciences Vétérinaires, University of Louvain-La-Neuve 3, Place Croix du Sud B 1348, Louvain La Neuve, Belgium |
| Abstract: | The effects of temperature and exposure time to vitrification solutions on In vitro survival of mouse blastocysts were investigated. Blastocysts were first exposed for 10 min to vitrification Solution 1 (VS1) containing 10% glycerol-20% 1,2 propanediol in phosphate buffered saline (PBS), then to vitrification Solution 2 (VS2) with 25 % glycerol-25% 1,2 propanediol for various periods either at room temperature or at 4°C. At room temperature survival dropped quickly, while at 4°C an increase in survival was observed. It is concluded that the viability of mouse blastocyts after vitrification is dependent on the temperature and duration of equilibration in vitrification solutions. |
| Keywords: | mouse blastocysts cryopreservation vitrification cryoprotectants |
| 本文献已被 ScienceDirect PubMed 等数据库收录！ | |