A rapid and sensitive method for the analysis of carbohydrate components in glycoproteins using gas-liquid chromatography |
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| Authors: | M F Chaplin |
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| Institution: | Department of Biochemistry, University of Leeds, Leeds LS2 9JT, United Kingdom |
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| Abstract: | A rapid and simple gas-liquid chromatographic method for the determination of subnanomolar amounts of carbohydrates derived from glycoproteins is described. The procedure involves methanolysis in the presence of methyl acetate followed by removal of hydrogen chloride by coevaporation with t-butyl alcohol and trimethylsilylation. The method is also applicable to samples containing uronic acids and lipids. |
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| Keywords: | HPLC high-performance liquid chromatography GLC gas-liquid chromatography GLC-MS gas-liquid chromatography-mass spectroscopy |
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