| 樟子松突变丛生枝蛋白质的双向电泳分析 |
| |
| 引用本文： | 李慧玉,董京祥,姜静.樟子松突变丛生枝蛋白质的双向电泳分析[J].生物技术,2004,14(1):35-37. |
| |
| 作者姓名： | 李慧玉 董京祥 姜静 |
| |
| 作者单位： | 东北林业大学林学院,黑龙江,哈尔滨,150040 |
| |
| 摘 要： | 目的：研究樟子松突变丛生枝的发生机理。方法：对其正常枝和突变丛生枝进行蛋白质双向电泳，通过ImageMaster2-DE计算机分析系统对结果进行定性、定量分析。结果：共有59个蛋白点在表达的质和量上有变化，其中35个蛋白点在突变丛生枝2-DE图谱中表达量均高于正常枝的蛋白点，20个蛋白点在突变丛生枝2-DE图谱中表达量均低于正常枝的蛋白点，4个蛋白点在突变丛生枝图谱上发生缺失。结论：差异表达的蛋白点可能与突变丛生枝的发生有关。
|
| 关 键 词： | 樟子松 突变丛生枝 双向蛋白电泳 蛋白质 |
| 文章编号： | 1004-311X(2004)01-0035-03 |
| 修稿时间： | 2003年11月11 |
Analysis of Multiple Shoot of Mutant in Pinus sylvestris by Two-dimensional Electrophoresis |
| |
| LI Hui-yu,DONG Jing-xiang,JIANG Jing.Analysis of Multiple Shoot of Mutant in Pinus sylvestris by Two-dimensional Electrophoresis[J].Biotechnology,2004,14(1):35-37. |
| |
| Authors: | LI Hui-yu DONG Jing-xiang JIANG Jing |
| |
| Abstract: | Objective:To analyze mechanism of multiple shoot of mutant.Method:The two-dimensional electrophoresis(2-DE) was employed to compare the global protein patterns between normal and mutant.The 2-De gel maps were evaluated qualitatively and quantitatively with the Image Master 2-DE analyzer;Results:The 59 expressed proteins in the mutant were different in quality and quantity from those in the normal,in which 35 spots increased and 20 spots decreased in quantity.Furthermore,4 spots were missing in mutant;Conclusion:The difference of maps of two stages might relate to multiple shoot of mutant. |
| |
| Keywords: | Pinus sylvestris mutant shoot two-dimensional electrophoresis |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
