Influence of mitotic delay on the results of biological dosimetry for high doses of ionizing radiation |
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Authors: | A Heimers H J Brede U Giesen W Hoffmann |
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Institution: | Centre of Environmental Research and Technology (UFT), University of Bremen, Leobenerstrasse, 28359, Bremen, Germany. aheimers@uni-bremen.de |
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Abstract: | The purpose of this study was to systematically investigate how high doses of sparsely and densely ionizing radiations influence
the proliferation time of lymphocytes in short-term cultures and, consequently, the observed frequencies of dicentric and
centric ring chromosomes. Peripheral blood samples from five volunteers were irradiated with high doses of 200 kV X-rays and
with neutrons with a mean energy of <E
n>=2.1 MeV. First division metaphase cells were collected after different culture times of 48, 56, and 72 h and dicentrics,
centric ring chromosomes, and acentric fragments were determined. The data hint at considerable mitotic delay. The main increase
in the number of chromosome aberrations occurred between 48 and 72 h after an X-ray exposure and between 56 and 72 h after
neutron exposure. When the data were used for a calibration of aberration frequency versus dose, subsequent dose estimations
resulted, however, in comparable values. Thus, in spite of the influence of mitotic delay on observable chromosome aberrations,
at least for the radiation types investigated here, a culture time of 48 h is acceptable for biological dosimetry. |
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