An assay for human lymphocyte mitogenic factor: B-lymphocyte stimulation by a T-lymphocyte product. |
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| Authors: | B H Littman J R David |
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| Affiliation: | 1. Division of Immunology and Connective Tissue Diseases, Department of Medicine, Medical College of Virginia, Health Sciences Division, Virginia Commonwealth University, Richmond, Virginia, USA;2. The Robert B. Brigham Hospital, Harvard Medical School, Boston, Massachusetts, USA |
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| Abstract: | Human lymphocyte mitogenic factor (LMF) is produced only by E-rosette positive T-lymphocytes when purified cell populations are used. Production is antigen specific and requires the presence of monocytes during the first 24 hr of culture. However, LMF production or regulation of production by cells other than T-lymphocytes may occur as more LMF activity is produced when B-lymphocytes are added to a primary culture of T-lymphocytes. The molecular weight of LMF is estimated by gel filtration to be between 20,500 and 28,000. LMF is heat stable.The response to LMF by B- or T-lymphocytes without monocytes is non-specific and is independent of and unaffected by antigen. The B-lymphocyte response is quantitatively greater that the response of T-lymphocytes. B-lymphocytes are much more sensitive to small concentrations of LMF. |
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