| 萱草花粉微管蛋白的体外聚合及电镜观察 |
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| 引用本文： | 廖俊杰,吴英杰,阎隆飞.萱草花粉微管蛋白的体外聚合及电镜观察[J].云南植物研究,2005,27(6):649-652. |
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| 作者姓名： | 廖俊杰 吴英杰 阎隆飞 |
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| 作者单位： | 1. 中国农业大学生物学院植物生理生化国家重点实验室,北京,100094;广东轻工职业技术学院食品与生物工程系,广东,广州,510300
2. 中国农业大学生物学院植物生理生化国家重点实验室,北京,100094 |
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| 基金项目： | 国家自然科学基金资助(39730280) |
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| 摘 要： | 微管(microtubule)作为细胞骨架的主要成分,在植物体内,微管除决定细胞的形状外,还参与很多重要的细胞功能。但有关微管蛋白生物化学的研究绝大多数来自动物脑组织材料,对植物微管蛋白的研究除培养细胞外所知甚少,我们纯化了毫克数量的萱草(Hemer-ocallis fulvaL.)花粉微管蛋白,利用紫杉醇作为促进剂,在Mg2 、GTP等存在下体外聚合成功,并观察了其电镜下的形态。
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| 关 键 词： | 萱草 微管 微管蛋白 体外聚合 电镜观察 |
| 文章编号： | 0253-2700（2005）06-0649-04 |
| 收稿时间： | 2005-05-08 |
| 修稿时间： | 2005年5月8日 |
Polymerziton and Electron Microscopy of Tubulin of Pollens from Hemerocallis fulva in vitro |
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| LIAO Jun-Jie,WU Ying-Jie,YEN Lung-Fei.Polymerziton and Electron Microscopy of Tubulin of Pollens from Hemerocallis fulva in vitro[J].Acta Botanica Yunnanica,2005,27(6):649-652. |
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| Authors: | LIAO Jun-Jie WU Ying-Jie YEN Lung-Fei |
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| Institution: | 1 College of Biological Sciences, China Agricultural University, State Key Labratory of Plant Physiology and Biochemistry, Beijing 100094, China; 2 Department of Food and Blo-technology , Guangdong Light Industry Technologic College, Guangzhou 510300, China |
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| Abstract: | Microtubules are the components of the cytoskeleton in all eukaryotic cells.They are essential for a wide varieties of cellular functions.Most of our knowledge of biochemistry and pharmacology of tubulin has come from extensive studies on mammalian brain microtubule systems,very little information exists on the biochemical and pharmacological properties of plant tubulin and microtubules.We developed an efficient method to prepare enough tubulin in high purity from day lily (Hemerocallis fulva L.) pollen.Here we reported the ultrastructural morphology of microtubule polymerzied by induction of tubulin taxol in the presence of Mg 2 and GTP with transmission electron microscopy. |
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| Keywords: | Hemerocallis fulva Tubulin Microtubule Polymerziton Electron microscopy |
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