The use of antiserum with specific reactivity toward fat-cell surface antigen(s) to follow the progression of 3T3-L1 preadipocyte differentiationin vitro |
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| Authors: | Hedwig A. K. Plaas J. Stuart Woodhead Anthony Cryer |
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| Affiliation: | (1) Department of Biochemistry, University College, P.O. Box 78, CFL 1XL Cardiff, Wales, UK;(2) Department of Medical Biochemistry, Welsh National School of Medicine, Heath Park, CF4 4XN Cardiff, Wales, UK;(3) Present address: Biochemistry Department, The Mathilda & Terence Kennedy Institute of Rheumatology, 6 Bute Gardens, Hammersmith, W6 7DW London, UK |
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| Abstract: | Using an i n d i r e c t , labelled-second-antibody cellular immunoassay technique, an adipocyte-specific antiserum haS been investigated. Components of the antiserum were shown to bind to differentiated 3T3-L1 cells; the cellular capacity for binding increased progressively during the induced differentiation of these ceils in vitro. |
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