Determination of finasteride in human plasma by liquid–liquid extraction and high-performance liquid chromatography |
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Authors: | P Pt
ek J Macek J Klíma |
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Institution: | P. Ptá
ek, J. Macek,J. Klíma |
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Abstract: | A high-performance liquid chromatographic method for the quantitation of finasteride in human plasma is presented. The method is based on liquid–liquid extraction with hexane–isoamylalcohol (98:2, v/v) and reversed-phase chromatography with spectrophotometric detection at 210 nm. The mobile phase consists of acetonitrile–15 mM potassium dihydrogenphosphate (40:60, v/v). Clobazam is used as the internal standard. The limit of quantitation is 4 ng/ml and the calibration curve is linear up to 300 ng/ml. Within-day and between-day precision expressed by relative standard deviation is less than 5% and inaccuracy does not exceed 8%. The assay was used for pharmacokinetic studies. |
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Keywords: | Finasteride |
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