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Isolation and characterization of microsatellite loci in Portunus pelagicus (Crustacea: Portunidae)
Authors:E. S. Yap  E. Sezmis  J. A. Chaplin  I. C. Potter  P. B. S. Spencer
Abstract:Seven dinucleotide and one tetranucleotide microsatellite loci were isolated and characterized, using routine protocols, from the blue swimmer crab, Portunus pelagicus. These loci will be useful for investigating the population structure of this broadly distributed and economically important portunid. In particular, all eight loci were polymorphic in P. pelagicus collected from throughout Australia. In addition, the genotype frequencies at each locus in each population sample were usually not significantly different from those expected under Hardy–Weinberg equilibrium conditions. Six of the eight loci also showed moderate to high levels of polymorphism in an undescribed species of Portunus from northern Australia.
Keywords:microsatellite markers  Portunidae  Portunus pelagicus
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