Polymerase chain reaction primers for polymorphic microsatellite loci from the túngara frog Physalaemus pustulosus |
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Authors: | H. Pr hl,R. M. M. Adams,U. Mueller,S. Rand,M. J. Ryan |
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Affiliation: | H. Pröhl,R. M. M. Adams,U. Mueller,S. Rand,M. J. Ryan |
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Abstract: | We developed eight PCR?primer pairs of polymorphic microsatellite loci for the túngara frog Physalaemus pustulosus. Genomic libraries were enriched for one of four microsatellite repeat sequences (CAn, GAn, ATGn and TAGAn). Following characterization of microsatellite loci by sequencing, primers were designed and PCR conditions optimized. Microsatellite PCR‐amplification was tested in 37 frogs from 8 populations in Costa Rica and Panama. Primer sequences, PCR conditions, allelelic diversities and observed as well as expected heterozygosities in the screened populations are described. |
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Keywords: | anuran genotyping microsatellite marker PCR Physalaemus pustulosus primer |
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