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PCR‐based markers detect genetic variation at growth and immune function‐related loci in chinook salmon (Oncorhynchus tshawytscha)
Authors:Margaret F Docker  Daniel D Heath
Abstract:We developed seven polymerase chain reaction (PCR) based markers that detect genetic variation at loci related to growth (four) and immune function (three) in chinook salmon. One assay shows a length polymorphism following PCR; the others show restriction fragment length polymorphisms (PCR‐RFLP). Two alleles were detected in each assay, and the common alleles were found at frequencies of 0.67–0.95 in seven wild populations in British Columbia, Canada. These loci also amplified in other salmonid species. These markers, by detecting variation linked to genes with high fitness relevance, are expected to be useful in a range of theoretical and applied studies.
Keywords:growth hormone introns  insulin‐like growth factor  MHC‐B1  Oncorhynchus tshawytscha  PCR‐RFLP  transferrin
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