Doubled haploid production via anther culture in annual,winter type of caraway (<Emphasis Type="Italic">Carum carvi</Emphasis> L.) |
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Authors: | Iva Smýkalová Jr" target="_blank">Prokop ?mirousJr Michaela Kubo?iová Nikol Gasmanová Miroslav Griga |
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Institution: | Iva Smykalová, Prokop ?mirous Jr, Michaela Kubo?iová, Nikol Gasmanová and Miroslav Griga |
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Abstract: | Caraway (Carum carvi L.) is a traditional medicinal and spice cross-pollinated plant species. Although in vitro techniques are recently extensively
applied in plant breeding programmes, these are not commonly utilized in caraway. Therefore, based on the protocol for anther
culture in carrot (Daucus carota L., a closely related species of caraway in Daucaceae family), in vitro androgenesis in caraway has been studied with the
aim to produce completely homozygous inbred lines. Various induction conditions, such as temperature pretreatments, carbon
sources and combination of growth regulators in a culture medium as well as the effect of genotype on in vitro androgenesis
were examined. Ten breeding lines of winter caraway representing third generation of forced (artificial) self-pollination
were used as donor plant material. Cultured anthers produced embryogenic calli, and subsequently two types of regenerated
plants were obtained, namely haploids with evident microspore origin, and diploids which may represent somatic (anther wall)
regenerants or spontaneous doubled haploids. The ploidy status of regenerated plants was determined by flow cytometry. This
is the first report on androgenic doubled haploid production in caraway. |
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Keywords: | Anther culture Carum carvi L Doubled haploids Embryogenesis Plant regeneration |
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