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毛竹基因组大小测定
引用本文:李潞滨,武静宇,胡陶,杨学文,彭镇华.毛竹基因组大小测定[J].植物学通报,2008,25(5):574-578.
作者姓名:李潞滨  武静宇  胡陶  杨学文  彭镇华
作者单位:1. 中国林业科学研究院林业研究所,国家林业局林木培育重点实验室,北京,100091
2. 中国林业科学研究院林业研究所,国家林业局林木培育重点实验室,北京,100091;河北大学生命科学学院,保定,071002
3. 国际竹藤网络中心,国家林业局竹藤科学与技术重点开放实验室,北京,100102
基金项目:林业公益性行业科研专项基金,引进国际先进农业科技计划(948计划)
摘    要:毛竹(Phyllostachys edulis)属禾本科(Poaceae)竹亚科(Bambusoideae)刚竹属(Phyllostachys),是我国分布和栽培面积最大的经济竹种,有着广泛的开发前景。本实验以水稻(Oryza sativa)为内标,用流式细胞仪对水稻和竹子样品的PI发射荧光强度进行测定,通过比较水稻与毛竹样品峰值的倍数关系,计算出毛竹的基因组大小。对24组样品进行重复测试,测得毛竹基因组大小为2075.025±13.08Mb,即2C DNA含量为4.24pg(以1pg DNA=0.978×10^9bp计算)。毛竹基因组大小测定为毛竹基因组文库的建立及其基因组学研究奠定了重要基础。

关 键 词:流式细胞仪  基因组大小  水稻  毛竹

Estimation of Genome Size of Moso Bamboo (Phyllostachys edulis)
Lubin Li,Jingyu Wu,Tao Hu,Xuewen Yang,Zhenhua Peng.Estimation of Genome Size of Moso Bamboo (Phyllostachys edulis)[J].Chinese Bulletin of Botany,2008,25(5):574-578.
Authors:Lubin Li  Jingyu Wu  Tao Hu  Xuewen Yang  Zhenhua Peng
Institution:Lubin Li, Jingyu Wu, Tao Hu, Xuewen Yang, Zhenhua Peng(1.Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China; 2.College of Life Sciences, Hebei University, Baoding 071002, China; 3.Bamboo and Rattan Science and Technology Laboratory, State Forestry Administration, International Center for Bamboo and Rattan, Beijing 100102, China)
Abstract:Moso bamboo (Phyllostachys edulis), a member of Bambusoideae (Bambusoideae), Gramineae (Poaceae), is one of the most important economic bamboo species in China and in the world. We aimed to estimate the genome size of moso bamboo by flow cytometry, using rice (Oryza sativa) as an internal reference. After comparing the peak values of the fluorescence intensity of 24 bamboo and rice samples, we calculated the genome size of moso bamboo to be about 2 075.025±13.08 Mb, or 2 C DNA about 4.24 pg (1 pg DNA = 0.978×10^9 bp). The results of this study will help in future research of the moso bamboo genome.
Keywords:flow cytometry  genome size  Oryza sativa  Phyllostachys edulis
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