Manganese superoxide dismutase from Biomphalaria glabrata |
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Authors: | Jung Younghun Nowak Thomas S Zhang Si-Ming Hertel Lynn A Loker Eric S Adema Coen M |
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Institution: | Department of Biology, University of New Mexico, Albuquerque, NM 87131, USA. |
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Abstract: | The investigation of the response of Biomphalaria glabrata snails to Echinostoma paraensei (digenea) at 2 days post-exposure by suppression subtractive hybridization yielded a partial sequence of the anti-oxidant enzyme manganese superoxide dismutase (MnSOD). Full-length MnSOD (669nt) from M line and BS-90 strains of B. glabrata differed by one synonymous nucleotide replacement. B. glabrata has 1-4 MnSOD loci (Southern hybridization). Both snail strains expressed MnSOD at equal baseline levels (quantitative PCR). Susceptible snails increased expression of MnSOD following infection with E. paraensei or Schistosoma mansoni, and expression was reduced in the incompatible combination (BS-90 B. glabrata and S. mansoni). Thus, MnSOD did not determine resistance or susceptibility for these parasites, but expression of MnSOD is consistent with its involvement in a stress response of B. glabrata. |
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Keywords: | Host-parasite immunobiology Subtraction suppressive hybridization Schistosoma mansoni Echinostoma paraensei |
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