Molecular Differentiation of Lactococcus lactis Subspecies lactis and cremoris Strains by Ribotyping and Site Specific-PCR |
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| Authors: | Pervin Basaran Nese Basaran Ibrahim Cakir |
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| Affiliation: | (1) Adnan Menderes University, Gazi Bulvari, Aydin, Turkey, TR;(2) Department of Food Science and Technology, Cornell University, Geneva, NY 14456, USA, US;(3) Food Engineering Department, Ankara University, Diskapi, Ankara, Turkey, TR |
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| Abstract: | Twenty-five strains of Lactococcus lactis subspecies lactis and subspecies cremoris obtained from dairy industry and environmental collections were examined by 16S RNA automated ribotyping profiles and site-specific PCR (S-PCR). By automated ribotyping, the majority of strains were classified in accordance with phenotypic characterization, with the exception of one lactis (220) and two cremoris (BO32 and 140) strains. A complete differentiation of subspecies lactis and cremoris in agreement with conventional phenotypic methods was achieved by S-PCR with a set of site-specific primer pairs (PR1, RM4, and F3) designed particularly from a deletion region found in subspecies cremoris, but not in lactis. Therefore, S-PCR with primers (PR1, RM4, and F3) is a rapid and very sensitive method for the distinction of lactis and cremoris subspecies in dairy production. Received: 19 June 2000 / Accepted: 17 July 2000 |
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