豚鼠气道及肺组织原癌基因表达与哮喘的相关研究

目的：为研究原癌基因在哮喘发病中的作用。方法：以卵白蛋白致敏豚鼠建立哮喘模型，用Ｄｏｔ－ｂｌｏｔ、Ｎｏｒｔｈ－ｅｒｎ－ｂｌｏｔ分子杂交及免疫组化技术，分别观察正常及哮喘发作后豚鼠气道上皮及肺组织中原癌基因ｃ－ｆｏｓ、ｃ－ｍｙｃ的表达水平。结果：正常豚鼠气道及肺组织中ｃ－ｆｏｓ及ｃ－ｍｙｃｍＲＮＡ无或很少表达，哮喘发作后豚鼠气道上皮及肺组织中ｃ－ｆｏｓ和ｃ－ｍｙｃｍＲＮＡ表达明显增强，３０ｍｉｎ达高峰，发作后４ｈ降至正常水平。地塞米松对ｃ－ｆｏｓ及ｃ－ｍｙｃｍＲ－ＮＡ有部分抑制作用。结论：原癌基因ｃ－ｆｏｓ及ｃ－ｍｙｃ在哮喘发病过程中可能起一定作用。

STUDY ON RELATIONSHIP BETWEEN PROTO ONCOGENE EXPRESSION OF c fos and c myc IN AIRWAY, LUNG TISSUE AND ASTHMA IN GUINEA PIGS

In order to investigate the mechanism of the attack of asthma, the authors examined the proto oncogene expression of c fos and c myc in the airway and lung tissue of the ovalbumin induced asthmatic guinea pigs by using Dot blot, Northern blot and immunohistochemical techniques. There was a baseline expression of c fos and c myc in the controls, whereas the mRNA expression of c fos and c myc increased much more significantly in asthmatic guinea pigs, it reached maximum at 30 min after the onset of asthma and it was in a time dependant manner. The inducement could be inhibited partially by dexamethasone. Immunohistochemical investigation showed that c fos and c myc was distributed mainly in epithelial cells of the airway. The staining was more intensive in the asthmatic guinea pigs than in the controls. The results suggest that the increase in proto oncogene expression of c fos and c myc may be related to the attack of asthma.