| 低氧对CRF,AVP和NE刺激体外培养腺垂体细胞生成cAMP的影响 |
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| 引用本文： | 陈志,杜继曾.低氧对CRF,AVP和NE刺激体外培养腺垂体细胞生成cAMP的影响[J].中国应用生理学杂志,1997,13(4):295-298. |
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| 作者姓名： | 陈志 杜继曾 |
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| 作者单位： | 中国科学院西北高原生物研究所(陈志)，浙江大学生物科学与技术系(杜继曾) |
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| 摘 要： | 本文探讨了ＣＲＦ，ＡＶＰ及ＮＥ对体外培养大鼠腺垂体细胞生成ｃＡＭＰ的作用。ＣＲＦ刺激体外培养大鼠腺垂体细胞内ｃＡＭＰ的生成，且浓度与效应正相关。ＡＶＰ未引起细胞内ｃＡＭＰ差异性变化（Ｐ＞０．５）。ＮＥ使培养腺垂体细胞内ｃＡＭＰ水平降低。低氧使ＣＲＦ刺激ｃＡＭＰ生成的作用降低。而ＡＶＰ及ＮＥ可能是通过其它胞内信息通路。
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| 关 键 词： | 低氧 促肾上腺皮质激素释放激素 培养腺垂体细胞 环磷酸腺苷 |
EFFECT OF CRF, AVP AND NE ON cAMP IN CULTURED RAT ANTERIOR PITUITARY CELLS DURING HYPOXIA |
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| Chen Zhi,Du Jizeng.EFFECT OF CRF, AVP AND NE ON cAMP IN CULTURED RAT ANTERIOR PITUITARY CELLS DURING HYPOXIA[J].Chinese Journal of Applied Physiology,1997,13(4):295-298. |
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| Authors: | Chen Zhi Du Jizeng |
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| Institution: | Northwestern Plateu Institute of Biology, Chinese Academy of Sciences, Xining. |
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| Abstract: | In the study, the effect of CRF(corticotropin releasing factor),AVP(argipressin) and NE(norepinephrine) on cAMP formation in cultured rat anterior pituitary cells in vitro was examined and the effect of cAMP formation between normoxia and hypoxia (8%O 2)was compared. CRF stimulated the formation of cAMP levels which was correlated with the concentration of CRF in medium.The cAMP levels in medium increased. The intracellular cAMP in cultured anterior pituitary cells was not changed by AVP. The concentration of intracellular cAMP was decreased by NE, but the content of extracellular cAMP was no changed. Hypoxia decreased the stimulating effects of CRF on the cAMP formation in cultured anterior pituitary cells. |
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| Keywords: | hypoxia corticotropin releasing factor(CRF) cultured rat anterior pituitary cells cAMP |
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