颈髓损伤后线粒体系列酶活性变化与线粒体功能的关系

为了探讨颈髓损伤后颈髓线粒体系列酶活性变化与线粒体功能的关系，采用Ａｌｅｎ法造成猫颈髓损伤，观察颈髓损伤后线粒体Ｃａ２＋，Ｍｇ２＋－ＡＴＰ酶、Ｎａ＋，Ｋ＋－ＡＴＰ酶、超氧化物歧化酶（ＳＯＤ）活性及线粒体呼吸功能的变化。结果显示：颈髓损伤后２ｈ至７２ｈ，Ｃａ２＋，Ｍｇ２＋－ＡＴＰ酶、Ｎａ＋，Ｋ＋－ＡＴＰ酶活性、ＳＯＤ活性明显降低，而线粒体呼吸控制率（ＲＣＲ）、磷氧比值（Ｐ／Ｏ）、氧化磷酸化效率（ＯＰＲ）也明显下降。表明颈髓损伤后Ｃａ２＋，Ｍｇ２＋－ＡＴＰ酶、Ｎａ＋，Ｋ＋－ＡＴＰ酶、ＳＯＤ活性与线粒体功能密切相关，提示颈髓线粒体的病理生理改变在颈髓损伤后继发性损害过程中起重要作用。

THE RELATIONSHIP BETWEEN ALTERATION OF MITOCHONDRIAL ENZYME ACTIVITIES AND MITOCHON DRIAL FUNCTION AFTER CERVICAL CORD INJURY

To investigate the relationship between mitochondrial enzyme activities and mitochondrial function after cervical cord injury, cervical cord model was prepared using Allen method in cats. Changes in mitochondrial Na , K ATPase, Ca 2 ,Mg 2 ATPase,superoxide dismutase (SOD) activities, contents of malondialdehyde (MDA) and mitochondrial function in cervical cord were observed by different biochemical methods. The results showed that mitochondrial Na , K ATPase, Ca 2 ,Mg 2 ATPase, SOD activities decreased; mitichondral respiratory control rate(RCR), P/O ratio and oxidative phosphorylation rate (OPR) were also depressed as compared with control group ( P <0 05),but MDA level significantly increased compard to control group ( P <0 01). The results demonstrate that there is close correlation, between mitochondrial ATPase, SOD activities and mitochondral function.suggesting that mitochondrial pathophysiological alteration plays important roles in the secondary damage after cervical cord injury.