Quantitative colorimetric and gas chromatoographic determination of arecaidine propargyl ester |
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Authors: | K Langer G Lambrecht U Moser E Mutschler J Kreuter |
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Institution: | aInstitut für Pharmazeutische Technologie, Biozentrum-Niederusel, Johann Wofgand Geothe-Universität, Marie-Curie Strasse 9, D-60439 Frankfurt am Main, Germany;bPharmakolofisches Institut für Naturwissenschafter, Biozentrum-Niederursel Wolfgang Goethe-Universität. Marie-Curie Strasse 9, D-60439 Frankfurt am Main, Germany |
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Abstract: | Arecaidine propargyl ester (APE) is a potent muscarinic agonist often used in pharmacological studies. To date, no sensitive quantitative analytical method for APE has been published. In this study, two methods for the quantitative determination of APE are compared: a colorimetric assay, based on the formation of the corresponding ferric(III)-hydroxamic acid complex, and a direct gas chromatographic method, using arecoline as the internal standard. The latter method was found to be more precise. The utility of the gas chromatographic assay was further demonstrated in a stability study of the drug in the biological fluid aqueous humor of rabbits. |
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Keywords: | Arecaidine propargyl ester |
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