The application of wheat microsatellites to identify disomic Triticum aestivum-Aegilops markgrafii addition lines |
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| Authors: | A Peil V Korzun V Schubert E Schumann W E Weber M S Röder |
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| Institution: | Institut für Pflanzenzüchtung und Pflanzenschutz, Martin-Luther-Universit?t Halle-Wittenberg, Berlinerstrasse 2, D-06188 Hohenthurm, Germany Fax:?034?602?154617 E-mail:?peil@landw.uni-halle.de, DE
Institut für Pflanzengenetik und Kulturpflanzenforschung, Corrensstrasse 3, D-06466 Gatersleben, Germany, DE
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| Abstract: | We describe the use of wheat microsatellites for the discrimination of Aegilops markgrafii chromosomes. Twenty out of eighty eight wheat microsatellites (WMS) tested were able to distinguish Triticum aestivum-Ae. markgrafii addition lines. Six, three, three, one and six of 18 WMS can be used as markers for single Ae. markgrafii chromosomes B, C, D, F and G, respectively. Addition line A is not available but additional bands, appearing only in Ae. markgrafii and the T. aestivum-Ae. markgrafii amphiploid and not in any of the available addition lines, indicate that three WMS detect markers for Ae. markgrafii chromosomes A. Addition line E could not be detected by any of the WMS markers applied, although the 20 WMS represented all
the homologous groups of wheat. All three WMS located on the short arm of group-2 chromosomes were located on Ae. markgrafii chromosome B; three of four WMS, located on the long arm of wheat group-2 chromosomes, were specific to Ae. markgrafii chromosome G and three of four WMS, specific to group-5 chromosomes, were markers for Ae. markgrafii chromosome C, indicating the homoeology of these wheat chromosome arms with the respective Ae. markgrafii chromosomes.
Received: 29 May 1997 / Accepted: 10 September 1997 |
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| Keywords: | Aegilops markgrafii Triticum aestivum Addition lines Chromosome markers Homoeology Wheat Wheat microsatellites |
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