MPrime: efficient large scale multiple primer and oligonucleotide design for customized gene microarrays |
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Authors: | Email author" target="_blank">Eric?C?RouchkaEmail author Abdelnaby?Khalyfa Nigel?GF?Cooper |
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Institution: | (1) Department of Computer Engineering and Computer Science, Speed School of Engineering, University of Louisville, Louisville, Kentucky, USA;(2) Department of Anatomical Sciences and Neurobiology, University of Louisville School of Medicine, Louisville, Kentucky, USA;(3) Bioinformatics Research Group, University of Louisville, Louisville, Kentucky, USA |
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Abstract: | Background Enhancements in sequencing technology have recently yielded assemblies of large genomes including rat, mouse, human, fruit
fly, and zebrafish. The availability of large-scale genomic and genic sequence data coupled with advances in microarray technology
have made it possible to study the expression of large numbers of sequence products under several different conditions in
days where traditional molecular biology techniques might have taken months, or even years. Therefore, to efficiently study
a number of gene products associated with a disease, pathway, or other biological process, it is necessary to be able to design
primer pairs or oligonucleotides en masse rather than using a time consuming and laborious gene-by-gene method. |
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