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Regulation of intracellular calcium concentration by nanosecond pulsed electric fields
Authors:Shaka S Scarlett  Peter F Blackmore  Juergen F Kolb
Institution:a Frank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, VA, USA
b Department of Physiological Sciences, Eastern Virginia Medical School, Norfolk, VA, USA
Abstract:Changes in Ca2+]i response of individual Jurkat cells to nanosecond pulsed electric fields (nsPEFs) of 60 ns and field strengths of 25, 50, and 100 kV/cm were investigated. The magnitude of the nsPEF-induced rise in Ca2+]i was dependent on the electric field strength. With 25 and 50 kV/cm, the Ca2+]i response was due to the release of Ca2+ from intracellular stores and occurred in less than 18 ms. With 100 kV/cm, the increase in Ca2+]i was due to both internal release and to influx across the plasma membrane. Spontaneous changes in Ca2+]i exhibited a more gradual increase over several seconds. The initial, pulse-induced Ca2+]i response initiates at the poles of the cell with respect to electrode placement and co-localizes with the endoplasmic reticulum. The results suggest that nsPEFs target both the plasma membrane and subcellular membranes and that one of the mechanisms for Ca2+ release may be due to nanopore formation in the endoplasmic reticulum.
Keywords:nsPEF  Membrane poration  Intracellular Ca2+  Jurkat cell  Fluo-4  Brefeldin A BODIPY®  
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