| 心肌组织特异性Isca1敲除造成新生大鼠心脏结构异常 |
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| 引用本文： | 杨辛兰,吕丹,张旭,董伟,陈炜,高珊,高凯,史旭东,马元武,张连峰.心肌组织特异性Isca1敲除造成新生大鼠心脏结构异常[J].中国实验动物学报,2019(3):286-290. |
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| 作者姓名： | 杨辛兰 吕丹 张旭 董伟 陈炜 高珊 高凯 史旭东 马元武 张连峰 |
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| 作者单位： | 中国医学科学院医学实验动物研究所;中国医学科学院医学实验动物研究所;中国医学科学院神经科学中心 |
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| 基金项目： | 中国医学科学院医学与健康科技创新工程(2016-I2M-1-015)~~ |
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| 摘 要： | 目的通过对心肌组织特异性Isca1敲除大鼠进行磁共振分析及病理学分析,探究心肌特异性敲除Isca1对大鼠心脏结构影响。方法繁育心肌组织特异性Isca1敲除大鼠,PCR技术鉴定大鼠基因型及基因敲除效率,对新出生0.5d及2.5d心肌组织特异性Isca1敲除大鼠进行核磁共振影像分析,对新出生2.5d心肌组织特异性Isca1敲除大鼠心肌组织进行H&E染色及透射电镜分析。结果心肌组织特异性Isca1敲除大鼠敲除效率大于78%;与野生型相比,0.5d心肌组织特异性Isca1敲除大鼠心脏未见显著扩张;2.5d心肌组织特异性Isca1敲除大鼠心脏右室呈扩张趋势;2.5d心肌组织特异性Isca1敲除大鼠肌纤维排列不齐,出现排列紊乱,无层次或极向,部分心肌纤维出现溶解断裂,肌节和Z线模糊,出现肌膜损伤,线粒体嵴断裂,肿胀明显。结论心肌组织特异性Isca1敲除造成新生大鼠心脏结构异常。
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| 关 键 词： | 心脏 新生鼠 Isca1 |
Isca1 myocardial conditional knockout results in cardiac structural abnormalities in newborn rats |
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| YANG Xinlan,LYU Dan,ZHANG Xu,DONG Wei,CHEN Wei,GAO Shan,GAO Kai,SHI Xudong,MA Yuanwu,ZHANG Lianfeng.Isca1 myocardial conditional knockout results in cardiac structural abnormalities in newborn rats[J].Acta Laboratorium Animalis Scientia Sinica,2019(3):286-290. |
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| Authors: | YANG Xinlan LYU Dan ZHANG Xu DONG Wei CHEN Wei GAO Shan GAO Kai SHI Xudong MA Yuanwu ZHANG Lianfeng |
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| Institution: | (Key Laboratory of Human Disease Comparative Medicine,National Health Commission of China (NHC),Institute of Laboratory Animal Science,Peking Union Medical College,Chinese Academy of Medical Sciences,Beijing 100021,China;Beijing Engineering Research Center for Experimental Animal Models of Human Diseases,Institute of Laboratory Animal Science,Peking Union Medical College,Chinese Academy of Medical Sciences,Beijing 100021;Neuroscience Center,Chinese Academy of Medical Sciences,Beijing 100730) |
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| Abstract: | YANG Xinlan;LYU Dan;ZHANG Xu;DONG Wei;CHEN Wei;GAO Shan;GAO Kai;SHI Xudong;MA Yuanwu;ZHANG Lianfeng(Key Laboratory of Human Disease Comparative Medicine,National Health Commission of China (NHC),Institute of Laboratory Animal Science,Peking Union Medical College,Chinese Academy of Medical Sciences,Beijing 100021,China;Beijing Engineering Research Center for Experimental Animal Models of Human Diseases,Institute of Laboratory Animal Science,Peking Union Medical College,Chinese Academy of Medical Sciences,Beijing 100021;Neuroscience Center,Chinese Academy of Medical Sciences,Beijing 100730) |
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| Keywords: | heart newborn rat Isca 1 |
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