应用流式细胞术测定17种中国野生蔷薇核DNA含量

以17种中国野生蔷薇为试材，采用改良的LB 01裂解液，以4种不同的标准植物——大豆（Glycine max Merr.‘Polanka’）、绿豆（Vigna radiata（L.） Wilczek）、番茄（Lycopersicon esculentum Miller）和玉米（Zea mays L.）为外标，以二倍体材料丽江蔷薇（Rosa×lichiangensis Yü et Ku）为内部参照，利用流式细胞术对其核DNA含量及染色体倍性进行检测，并采用常规染色体压片法验证倍性准确性。本研究首次检测了3个二倍体种——商城蔷薇（Rosa shangchengensis T.C.Ku）、广东蔷薇（Rosa kwangtungensis Yü et Tsai）和无刺刺梨（Rosa roxburghii f.inermis S.D.Shi），1个三倍体种——伞房蔷薇（Rosa corymbulosa Rolfe）和1个四倍体种——弯刺蔷薇（Rosa beggeriana Schrenk）的核DNA含量及基因组大小。结果表明，流式细胞术检测结果与常规染色体压片法结果一致，可对中国野生蔷薇的倍性研究进行补充。本研究结果可丰富中国蔷薇属植物的细胞遗传学背景资料并为繁育新品种提供理论依据。

Estimation of nuclear DNA content of 17 Chinese wild rose species by flow cytometry

Using four different standard plants (Glycine max Merr.‘Polanka’,Vigna radiata(L.) Wilczek,Lycopersicon esculentum Miller,and Zea mays L.) as outer calibrations and diploid taxon Rosa×lichiangensis Yü et Ku as the inner calibration,we estimated the nuclear genome sizes of 17 wild roses in China using flow cytometry based on the improved LB 01 buffer.Simultaneously,traditional chromosome tableting was performed to verify ploidy levels.The nuclear DNA contents and genome sizes of three diploid species (Rosa shangchengensis T.C.Ku,Rosa kwangtungensis Yü et Tsai and Rosa roxburghii f.inermis S.D.Shi),one triploid species (Rosa corymbulosa Rolfe),and one tetraploid species (Rosa beggeriana Schrenk) were reported in this study for the first time.The determined ploidy levels based on flow cytometry were consistent with those obtained by the traditional chromosome tableting technique.These findings enrich the cytogenetic resources of Rosa L.in China and offer a basis for the breeding of new strains.