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| 一种改良的大豆线粒体DNA提取方法 | |
| 引用本文： | 李文跃,林春晶,丁孝羊,韩亚丽,彭宝,赵丽梅,张春宝.一种改良的大豆线粒体DNA提取方法[J].植物科学学报,2018,36(6):888-892. |
| 作者姓名： | 李文跃 林春晶 丁孝羊 韩亚丽 彭宝 赵丽梅 张春宝 |
| 作者单位： | 1. 吉林农业大学农学院, 长春 130018; 2. 吉林省农业科学院大豆研究所, 长春 130033; 3. 大豆国家工程研究中心, 长春 130033 |
| 基金项目： | 吉林省科技厅重大科技攻关项目（20170201001NY）；国家自然科学基金项目（31601322）；吉林省农业科技创新工程项目（CXGC2017JQ004，CXGC2017TD002，C7208000409）。 |
| 摘 要： | 以大豆(Glycine max (L.) Merrill)黄化苗为材料,通过优化提取线粒体DNA(mtDNA)时差速离心过程中的离心力和离心时间,以及纯化过程中设置不同的蔗糖密度梯度和裂解液浓度,结合高盐法去除蛋白质,改良大豆mtDNA的提取方法。结果表明,该方法提取的mtDNA浓度和纯度较高,无叶绿体和核基因组DNA的污染,可用于后续大豆线粒体基因组的相关研究。 |
| 关 键 词： | 大豆 线粒体DNA 纯化线粒体 |
| 收稿时间： | 2018-06-14 |
An improved method to extract Glycine max mitochondrial DNA |
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| Li Wen-Yue,Lin Chun-Jing,Ding Xiao-Yang,Han Ya-Li,Peng Bao,Zhao Li-Mei,Zhang Chun-Bao.An improved method to extract Glycine max mitochondrial DNA[J].Plant Science Journal,2018,36(6):888-892. | |
| Authors: | Li Wen-Yue Lin Chun-Jing Ding Xiao-Yang Han Ya-Li Peng Bao Zhao Li-Mei Zhang Chun-Bao |
| Institution: | 1. College of Agriculture, Jilin Agricultural University, Changchun 130018, China; 2. Soybean Research Institute, Jilin Academy of Agricultural Sciences, Changchun 130033, China; 3 National Engineering Research Center for Soybean, Changchun 130033, China |
| Abstract: | In this study, yellowing Glycine max(L.) Merrill seedlings were used to extract mitochondrial DNA(mtDNA) by optimizing centrifugal force and time during differential centrifugation. Furthermore, establishing different sucrose density gradients and lysate concentrations during purification, combined with high salt removal protein, improved the extraction method of soybean mtDNA. Results showed that the concentration and purity of mtDNA extracted by this method were high, with no chloroplast or nuclear genomic DNA contamination, the mtDNA could be used for subsequent research on soybean mitochondrial genome. |
| Keywords: | Glycine max Mitochondrial DNA Purification of mitochondria |
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