油棕DGAT2基因启动子克隆及表达的组织特异性研究

以油棕(Elaeis guineensis Jacq.)叶片基因组DNA为模板,克隆获得长度为1035 bp的二酰甘油酰基转移酶基因(DGAT2)的启动子区序列。序列分析结果表明,DGAT2基因启动子含有大量光反应元件、激素响应元件及部分转录因子结合位点。本研究同时构建了DGAT2基因启动子和GUS基因植物融合表达载体,通过蘸花法侵染拟南芥(Arabidopsis thaliana L.),并对转基因拟南芥中GUS基因表达的特异性进行了分析。结果显示,GUS基因在拟南芥各组织中均有表达,但没有明显的组织特异性;荧光定量PCR分析结果表明DGAT2在油棕不同器官中的转录水平存在明显差异。

Cloning and tissue-specific expression analysis of type2 diacylglycerol acyltransferase gene (DGAT2) promoter in Elaeis guineensis

In this study, the promoter region of the DGAT2 gene was isolated and characterized from oil palm (Elaeis guineensis Jacq.). Based on genomic DNA sequence analysis, a 1035 bp promoter region was amplified by PCR. Several functional elements, such as TATA-box and CAAT-box, were identified from the obtained sequence by alignment in the database. To verify the tissue specificity of the GUS gene in different tissues, we constructed the DGAT2 promoter expression vector that contained the GUS gene and transformed the vector into Arabidopsis thaliana via the floral dip method. GUS staining of transgenic A.thaliana indicated that the DGAT2 promoter can drive gene expression without significant tissue specificity but showed obvious discrepancies in different organs by real-time PCR.