Characterization of sacbrood virus and its infection in Apis cerana in the Qiandao Lake region in Zhejiang Province,China |
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| Affiliation: | 1. Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, PR China;2. Key Laboratory for Insect Pollinator Biology of the Ministry of Agriculture and Rural Affairs, Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, PR China;1. Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Key Lab of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base, Nanjing 210014, China;2. College of Life Sciences, Huaibei Normal University, Huaibei 235000, China;3. Education Ministry Key Laboratory of Integrated Management of Crop Diseases and Pests, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China;4. Institute of Plant Protection, Jiangxi Academy of Agricultural Science, Nanchang 330200, China;1. The BCPH Unit of Molecular Physiology, Department of Chemistry, Biology and Marine Science, Faculty of Science, University of the Ryukyus, Nishihara, Okinawa 903-0213, Japan;2. Center for Research Advancement and Collaboration, University of the Ryukyus, Nishihara, Okinawa 903-0213, Japan;3. Japan Butterfly Conservation Society, Shinagawa, Tokyo, Japan;4. Institute for Integrated Radiation and Nuclear Science, Kyoto University, Kumatori, Osaka 590-0494, Japan;1. Ojeong Eco-Resilience Institute, Korea University, Seoul 02841, Republic of Korea;2. Department of Environmental Science and Ecological Engineering, Korea University, Seoul 02841, Republic of Korea;3. Institute of Environment and Ecology, OJERI, Korea University, Seoul 02841, Republic of Korea;1. Department of Plant Medicine, College of Agriculture, Life and Environment Sciences, Chungbuk National University, Cheongju 28644, Republic of Korea;2. Plant Quarntine Technology Center, Animal and Plant Quarantine Agency, Gimcheon 39660, Republic of Korea;1. Department of Applied Biology, College of Agriculture and Life Sciences, Chonnam National University, Gwangju, Republic of Korea;2. Herbal Medicine Resources Research Center, Korea Institute of Oriental Medicine, Naju, Republic of Korea;3. Department of Plant Quarantine, Animal and Plant Quarantine Agency, Gimcheon-si, Republic of Korea |
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| Abstract: | Chinese sacbrood virus (CSBV) is a disastrous virus that fatally threatens honeybees (Apis cerana) in China. Here, we identified the complete genome of CSBV using the rapid amplification of cDNA ends (RACE) method and compared the obtained sequences with those of other SBVs through multiple sequence alignment. The results showed that the full-length of CSBV genome from Zhejiang Province (CSBV-ZJ) is 8824 bp and contains a single, large open reading frame (ORF) that encodes 2842 amino acids flanked by 190 nucleotides (nts) of the 5′-untranslated region (UTR) and 108 nts of the 3′-UTR. The multiple genome comparison showed that SBVs have five conserved domains and that CSBV-ZJ belong to SBV type II. The phylogenetic analysis demonstrated that the CSBV-ZJ strain was similar to the CSBV from Fuzhou (CSBV-FZ) strain. Colonies collected from most of the sampling sites in the area surrounding Qiandao Lake were carriers of CSBV. The haplotypes of the VP1 gene formed a network with radiated and reticular structures. In conclusion, this characterization of the CSBV-ZJ genome will help us understand the SBV prevalence mechanism and aid the protection of honeybees. |
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| Keywords: | CSBV Capsid protein Complete genome sequence Phylogenetic analysis |
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