Spore formation and karyological characterization of basidiosporogenesis,meiosis, post-meiotic and nuclear migration mitosis in Coprinus comatus |
| |
| Institution: | 1. Graduate School of Sustainability Science, Tottori University, 4-101 Koyama-cho Minami, Tottori, 680-8553, Japan;2. Faculty of Agriculture, Tottori University, 4-101 Koyama-cho Minami, Tottori, 680-8553, Japan;1. Graduate school of Infection Control Sciences, Kitasato University, 5-9-1, Shirokane, Minato-ku, Tokyo, 108-8641, Japan;2. Kitasato Institute for Life Sciences, Kitasato University, 5-9-1, Shirokane, Minato-ku, Tokyo, 108-8641, Japan;1. Department of Plant Protection, Faculty of Plant Production, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran;2. Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran;3. Department of Biology, Rutgers University, New Brunswick, NJ, USA;4. Department of Biotechnology & Plant Breeding, Faculty of Plant Production, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran;1. Graduate School, Khon Kaen University, Khon Kaen, 40002, Thailand;2. Biology Program, Faculty of Science, Buriram Rajabhat University, Buriram, 31000, Thailand;3. Department of Biochemistry, Faculty of Science, Khon Kaen University, Khon Kaen, 40002, Thailand;4. Department of Biochemistry and Biotechnology, Faculty of Agriculture, Tottori University, Tottori, 680-8553, Japan;5. Department of Microbiology, Faculty of Science, Khon Kaen University, Khon Kaen, 40002, Thailand;1. Department of Chemistry of Natural and Microbial Products, National Research Centre, Dokki, Cairo, 12622, Egypt;2. Department of Genetics and Cytology, National Research Centre, Dokki, Cairo, 12622, Egypt;3. Department of Microbiology, Faculty of Science, Ain Shams University, Abbassia, Cairo, 11566, Egypt;1. Faculty of Agriculture, Tottori University, 4-101, Koyama, Tottori, 680-8553, Japan;2. Department of Bioscience and Food Production Science, Interdisciplinary Graduate School of Science and Technology, Shinshu University, 8304, Minami-minowa, Nagano, 399-4598, Japan;3. Department of Bioscience and Biotechnology, Faculty of Agriculture, Shinshu University, 8304, Minami-minowa, Nagano, 399-4598, Japan;4. Division of Terrestrial Ecosystem, Institute of Mountain Science, Shinshu University, 8304, Minami-minowa, Nagano, 399-4598, Japan;5. Department of Fungal Symbiotic Science & Resource Utilization Technology, Research Center for Fungal and Microbial Dynamism, Shinshu University, 8304, Minami-minowa, Nagano, 399-4598, Japan;6. Nara Forest Research Institute, 1, Kibi, Takatori, Takaichi-gun, Nara, 635-0133, Japan;7. Fungus/Mushroom Resource and Research Center, Faculty of Agriculture, Tottori University, 4-101, Koyama, Tottori, 680-8553, Japan;1. State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, No. 3 Park 1, Beichen West Road, Chaoyang District, Beijing, 100101, PR China;2. Microbiome Research Center, Moon (Guangzhou) Biotech Ltd., Guangzhou, 510535, PR China |
| |
| Abstract: | In Coprinus comatus, we sampled and observed different maturity stages of basidium to find the possible causes of binucleate basidiospores by microscopy using the HCI-Giemsa and DAPI staining methods. In basidium, following karyogamy, a single nucleolus was observed. We found evidence suggesting post-meiotic mitosis following nuclear migration in the spores. Post-meiotic mitosis occurred in the basidiospores, resulting in four binucleate basidiospores. This indicates that the basidiospore is a monokaryon, and that C. comatus shows the same pattern of basidiospore formation as Coprinopsis cinerea. This type of nuclear behavior was defined as pattern D, one of six distinct patterns (pattern A–F) of nuclear behavior during basidiosporogenesis have been described for basidiomycetes by Campos and Costa in 2010. |
| |
| Keywords: | Basidiospore Basidium Monokaryon |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
