Identification of DNA Aptamers toward Epithelial Cell Adhesion Molecule via Cell-SELEX |
| |
| Authors: | Ji Won Kim Eun Young Kim Sun Young Kim Sang Kyung Byun Dasom Lee Kyoung-Jin Oh Won Kon Kim Baek Soo Han Seung-Wook Chi Sang Chul Lee Kwang-Hee Bae |
| |
| Affiliation: | 1.Research Center for Integrated Cellulomics, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-806, Korea;2.Department of Functional Genomics, University of Science and Technology (UST) of Korea, Daejeon 305-806, Korea;3.Medical Proteomics Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-806, Korea |
| |
| Abstract: | The epithelial cell adhesion molecule (EpCAM, also known as CD326) is a transmembrane glycoprotein that is specifically detected in most adenocarcinomas and cancer stem cells. In this study, we performed a Cell systematic evolution of ligands by exponential enrichment (SELEX) experiment to isolate the aptamers against EpCAM. After seven round of Cell SELEX, we identified several aptamer candidates. Among the selected aptamers, EP166 specifically binds to cells expressing EpCAM with an equilibrium dissociation constant (Kd) in a micromolar range. On the other hand, it did not bind to negative control cells. Moreover, EP166 binds to J1ES cells, a mouse embryonic stem cell line. Therefore, the isolated aptamers against EpCAM could be used as a stem cell marker or in other applications in both stem cell and cancer studies. |
| |
| Keywords: | aptamer EpCAM SELEX stem cell marker |
|
|
