Down-regulation of hepatic G-6-Pase expression in hyperglycemic rats: Intervention with biogenic gold nanoconjugate |
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Authors: | Sengani Manimegalai Shahid Mahboob Khalid A. Al-Ghanim Fahad Al-Misned Marimuthu Govindarajan Krishnan Anbarasu Vijayarangan Devi Rajeswari |
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Affiliation: | 1. Department of Bio-Medical Sciences, School of BioSciences and Technology, Vellore Institute of Technology (VIT), Vellore 632 014, Tamil Nadu, India;2. Department of Zoology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia;3. Unit of Vector Control, Phytochemistry and Nanotechnology, Department of Zoology, Annamalai University, Annamalainagar 608 002, Tamil Nadu, India;4. Department of Zoology, Government College for Women (Autonomous), Kumbakonam 612 001, Tamil Nadu, India;5. Department of Bioinformatics, School of Life Sciences, Vels Institute of Science, Technology & Advanced Studies, Pallavaram, Chennai 600 117, Tamil Nadu, India |
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Abstract: | Chronic diabetes extensively complicates the glucose metabolism to onset and progress the complication. Concurrently, several contemporary medicines, especially organo-metallic formulations, are emerging to treat hyperglycemia. The current study aims to emphasize the gold nanoparticles (GNPs) potential for glucose metabolism regulation in Streptozotocin (STZ) induced diabetes. Quantitative real-time polymerase chain reaction (RT-PCR) was carried out to detect the mRNA expression of Glucose transporters 2 (GLUT2), Glucokinase (GK) and Glucose 6 Phosphatase (G-6-Pase). The study shows remarkable results such as the prognostic effect of GNPs in reinforcing the repression of enzyme complex G-6-Pase about 13.3-fold when compared to diabetes control. Also, molecular docking studies showed significant inhibition of G-6-Pase by the terpenoid ligands with alpha and beta amyrin from leaf extract of Couroupita guianensis. Thus the study explored the novel mechanism of G-6-Pase downregulated by GNPs intervention that majorly contributes to the regulation of circulatory glucose homeostasis during diabetes. |
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Keywords: | Diabetes Glucose 6 phosphatase Gold nanoparticles Quantitative RT-PCR |
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