Anticancer activities of selected Emirati Date (Phoenix dactylifera L.) varieties pits in human triple negative breast cancer MDA-MB-231 cells |
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Authors: | Muhammad Nasir Khan Khattak Abdallah Shanableh Muhammad Iftikhar Hussain Amir Ali Khan Muhammad Abdulwahab Wameed Radeef Mohammad Harb Samreen |
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Affiliation: | 1. Department of Applied Biology, College of Sciences, University of Sharjah, Sharjah, United Arab Emirates;2. Human Genetics and Stem Cells Research Group, Research Institute of Sciences & Engineering (RISE), University of Sharjah, Sharjah, United Arab Emirates;3. Research Institute of Science and Engineering (RISE), University of Sharjah, Sharjah, United Arab Emirates;4. Department of Civil and Environmental Engineering, College of Engineering, University of Sharjah, United Arab Emirates;5. Department of Plant Biology and Soil Science, University of Vigo, Vigo 36310, Pontevedra, Spain;6. Department of Civil and Environmental Engineering, Carleton University, Ottawa, Canada;7. College of Pharmacy, University of Sharjah, United Arab Emirates;8. Sharjah Institute of Medical Research, University of Sharjah, Sharjah, United Arab Emirates |
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Abstract: | The date palm (Phoenix dactylifera L.) is an important fruit crop with significant pharmaceutical potential. Little data are available on comparative pharmaceutical importance of the date pits. We designed this study to assess the antitumorigenic effects of date palm pits extracts from different Emiratis varieties. We used MDA-MB-231 cells derived from triple negative breasts cancer tissues as a model. We found that out of the 17 date pits extracts from 6 Emiratis varieties, three (Khalas extract in water + acetone (1:1), Abu-Maan extract in MeOH + Chloroform (1:1) and Mabroom extract in water + acetone (1:1)) were found effectively cytotoxic and changed morphology of cells in dose and time dependent manner. We found the maximum effect at 2.5 mg/mL concentration at 72 h. We calculated IC50 values for these varieties at 24 h. IC50 values for Khalas, Abu-Maan and Mabroom were 0.982 mg/mL, 1.149 mg/mL and 2.213 mg/mL respectively. We treated the cells with IC50 values of extracts and observed changes in protein profile using human kinase array kit. After analyzing the results, we suggest that EGFR/ERK/FAK pathway, eNOS and src family proteins are targets of these extracts. We conclude that date pits extracts can be a possible therapeutic agent against cancer and we suggest further studies. |
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Keywords: | Anticancer activity MDA-MB-231 cells Pits extracts EGFR/ERK/FAK |
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