Comparative transcriptome analysis of wing discs from Bombyx mori and Bombyx mandarina |
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| Affiliation: | 1. School of Biology and Basic Medical Sciences, Soochow University, Suzhou, Jiangsu 215123, China;2. National Engineering Laboratory for Modern Silk, Soochow University, Suzhou, Jiangsu 215123, China;3. School of Studies in Zoology, Jiwaji University, Gwalior 474011, India;1. Department of Biology, Brigham Young University, 4102 LSB, Provo, UT 84602, USA;2. The New Zealand Institute for Plant & Food Research Limited, PO Box 92169, Mt Albert, Auckland, New Zealand;3. Monte L. Bean Museum, Brigham Young University, Provo, UT 84602, USA;1. School of Karst Science, Guizhou Normal University/ State Engineering Technology Institute for Karst Desertification Control, Guiyang, Guizhou 550001, China;2. Guizhou Provincial Key Laboratory for Rare Animal and Economic Insect of the Mountainous Region, Guiyang University, Guiyang, Guizhou 550005, China;1. USDA-ARS, US Pacific Basin Agricultural Research Center, Hilo, HI 967820, USA;3. Horticultural and Herbal Crop Environment Division, National Institute of Horticultural and Herbal Science, Wanju 55365, Republic of Korea;4. Forest Insect Pests and Disease Division, National Institute of Forest Science, Seoul 02455, Republic of Korea;1. Department of Biological Sciences, University of Memphis, 3700 Walker Avenue, Memphis, TN 38152, USA;2. Center for Biodiversity Research, University of Memphis, 3700 Walker Avenue, Memphis, TN 38152, USA;3. Plant Quarantine Technology Center, APQA, MAFRA, 167, Yongjeon-ro Gimcheon-si Gyeongsanbuk-do 39660 Republic of Korea;4. Senckenberg Deutsches Entomologisches Institut, Eberswalder Straße 90, 15374 Müncheberg, Germany;5. Insect Biosystematics Laboratory, Seoul National University, Seoul 151-921, Republic of Korea;6. Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Republic of Korea;7. Research Institute for Agricultural and Life Sciences, Seoul National University, Seoul 151-921, Republic of Korea |
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| Abstract: | The insect wing is developed from the wing imaginal disc which is designed from the embryonic ectoderm. To get insight into gene expression profiles in wing discs of Bombyx mori during metamorphosis, we compared the gene expression in the wing between B. mori and B. mandarina moth through RNA-seq. Out of total valid reads identified from the 5th day of 5th instar larvae of silkworm (L5), 7th day of pupae (P7), 1st day of moth (M1) and 1st day of wild silkworm moth (WM1), 20,092,004, 29,251,647, 24,654,695 and 19,753,089 reads were mapped to the mRNA reference sequences of silkworm, respectively. 9229, 7048, 9268 and 6701 differentially expressed genes (DEGs) were respectively recorded in P7 vs L5, M1 vs P7, M1 vs L5 and WM1 vs M1. Further, the peroxisome, ribosome, endocytosis and oxidative phosphorylation pathways were significantly regulated in the metamorphosis of the silkworm. Our study identified 16 orthologous genes with a positive selection from M1, which might be subjected to artificial selection in the domestication of B. mori and would play vital roles in the flight of B. mandarina. |
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| Keywords: | Wing disc Metamorphosis Wing |
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