Morphological and molecular analyses of Leafhopper,Amrasca biguttula (Ishida) (Hemiptera: Cicadellidae) infesting eggplant (Solanum melongena L.) in Luzon Island,Philippines |
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| Institution: | 1. Genetics Laboratory, Institute of Plant Breeding, College of Agriculture and Food Science, University of the Philippines, Los Baños, Laguna 4031, Philippines;2. Entomology Laboratory, Institute of Plant Breeding, College of Agriculture and Food Science, University of the Philippines, Los Baños, Laguna 4031, Philippines;1. Entomology Laboratory, Department of Zoology, University of Calcutta, 35 Ballygunge Circular Road, Kolkata 700 019, India;2. Cell and Molecular Therapeutics Laboratory, Department of Biochemistry and Biotechnology, Oriental Institute of Science and Technology, Vidyasagar University, Midnapore 721102, West Bengal, India;1. Institute of Plant Protection, Guizhou Academy of Agricultural Sciences, Guiyang 550006, China;2. Southern Insect Management Research Unit, Agricultural Research Service, United States Department of Agriculture, Stoneville, MS 38776, USA;3. Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China |
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| Abstract: | Eggplant (Solanum melongena L.) is the leading vegetable grown and consumed in the Philippines, and the eggplant leafhopper is one of its most important insect pests. Nine local populations of leafhopper infesting eggplants were collected from major eggplant growing areas in Luzon Island to determine if these populations are constituted singly or by different species and to assess the level of variability among them. Morphological analysis of the adult male abdominal and genital traits and partial mitochondrial COI gene sequence identified the eggplant leafhopper populations in Luzon Island as belonging to Amrasca (=Sundapteryx) biguttula (Ishida) (Hemiptera:Cicadelliae). Observed male specimens exhibited modifications in the pregenital abdominal tergites VII and VIII that were previously reported to not be found in other Amrasca species. Principal Component Analysis of four morphometric genital traits revealed high similarity among the leafhopper populations regardless of eggplant production areas. Partial COI sequence analysis idenfitied 11 sequence variants (haplotypes), with one predominant haplotype. Overall, the leafhopper populations from Luzon Island exhibited moderate haplotype diversity and low nucleotide diversity, indicating low genetic diversity. Topologies from a maximum likelihood tree indicate all eleven haplotypes cluster in a single clade with other A. biguttula infesting cotton and potato in India, but the Philippine population forms a separate subgroup although with weak bootstrap support. Further analysis with the Generalized Mixed Yule Coalescent (GMYC) method classified the different haplotypes into a single GMYC entity. |
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| Keywords: | Cytochrome oxidase I Leafhopper Mitochondrial DNA Morphology COI"} {"#name":"keyword" "$":{"id":"k0035"} "$$":[{"#name":"text" "_":"cytochrome oxidase I DNA"} {"#name":"keyword" "$":{"id":"k0045"} "$$":[{"#name":"text" "_":"deoxyribonucleic acid LH"} {"#name":"keyword" "$":{"id":"k0055"} "$$":[{"#name":"text" "_":"leafhopper PCR"} {"#name":"keyword" "$":{"id":"k0065"} "$$":[{"#name":"text" "_":"polymerase chain reaction |
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