Molecular analysis of mitochondrial cytochrome oxidase I gene of Aedes aegypti L. mosquitoes |
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| Institution: | 1. Maharashtra Hybrid Seeds Company Private Limited (MAHYCO), Jalna-Aurangabad Road, Dawalwadi, Badnapur, P.O. Box – 76, Jalna 431 203, Maharashtra State, India;2. Gangabishan Bhikulal Investment and Trading Limited (GBIT), Jalna-Aurangabad Road, Dawalwadi, Badnapur, P.O. Box – 76, Jalna 431 203, Maharashtra State, India;1. Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Key Lab of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base, Nanjing 210014, China;2. College of Life Sciences, Huaibei Normal University, Huaibei 235000, China;3. Education Ministry Key Laboratory of Integrated Management of Crop Diseases and Pests, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China;4. Institute of Plant Protection, Jiangxi Academy of Agricultural Science, Nanchang 330200, China;1. School of Horticulture and Plant Protection & Institute of Applied Entomology, Yangzhou University, Yangzhou 225009, China;2. Joint International Research Laboratory of Agriculture and Agri-Product Safety, Ministry of Education, Yangzhou University, Yangzhou, China;1. Integrated Pest Management Laboratory, Department of Entomology, University of Agriculture, Faisalabad, Pakistan;2. Department of Entomology, University of Agriculture, Faisalabad, Depalpur Campus, Okara, Pakistan;3. Key Lab of Molecular Biology of Crop Pathogens and Insects, Institute of Insect Sciences, College of Agriculture & Biotechnology, Zhejiang University, Hangzhou 310058, PR China;4. Institute of Geography, Fujian Normal University, 350007, China;5. College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, 518060, PR China;6. Insect Biodiversity and Conservation Group, Department of Entomology, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi, Pakistan;7. Research Center for Advanced Materials Science (RCAMS), King Khalid University, P.O. Box 9004, Abha 61413, Saudi Arabia;8. Unit of Bee Research and Honey Production, Faculty of Science, King Khalid University, P.O. Box 9004, Abha 61413, Saudi Arabia;9. Biology Department, Faculty of Science, King Khalid University, P.O. Box 9004, Abha 61413, Saudi Arabia;10. Department of Botany, Government College University Faisalabad, Pakistan;1. Department of Applied Biology, College of Agriculture and Life Sciences, Chonnam National University, Gwangju, Republic of Korea;2. Herbal Medicine Resources Research Center, Korea Institute of Oriental Medicine, Naju, Republic of Korea;3. Department of Plant Quarantine, Animal and Plant Quarantine Agency, Gimcheon-si, Republic of Korea |
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| Abstract: | Aedes aegypti is the most important arboviral vector worldwide. Recent studies reported that genetic variations and gene flow among same mosquito species is responsible for different disease transmission rate. Hence, to understand the relationship between genetic diversity and disease transmission potential, study on genetic variations among mosquito populations is essential. The aim of present study was to investigate the genetic variations of Ae. aegypti targeting COI gene from nine villages of Jalna District, Maharashtra and three laboratory strains originated from Aurangabad, Delhi and transgenic OX513A strain imported from OXITEC, UK. OX513A strain consists of a self-limiting dominant lethal gene construct intended for its use in suppression of Ae. aegypti population by sustained male adult releases in the environment. Mosquito eggs from field and laboratory strains were reared to adults and identified on the basis of morphological characteristics followed by COI gene sequence. Result of MSA and haplotype analysis revealed low genetic variations among field samples and Aurangabad strain, belonged to two haplotypes (H1 and H2) except Ramkheda village represented by separate haplotype H3. Other laboratory DEL strain and transgenic OX513A have great genetic variability to all isolates and have a separate haplotypes H4 and H5. Similar results were observed in phylogenetic analysis. Our observation of phylogenies revealed close relationship among the DEL and transgenic strain OX513A with few Indian and worldwide isolates. The information on genetic variability of mosquito population could help to understand and design the strategies for risk mitigation and effective implementation of new vector control tools like genetically modified mosquitoes. |
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| Keywords: | Haplotype Phylogenetic OX513A |
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