Physisporinus is an important mycorrhizal partner for mycoheterotrophic plants: Identification of mycorrhizal fungi of three Yoania species |
| |
| Institution: | 1. Tsukuba Botanical Garden, National Museum of Nature and Science, 4-1-1 Amakubo, Tsukuba, Ibaraki, 305-0005, Japan;2. Kyushu Research Center, Forestry and Forest Products Research Institute, 4-11-16 Kurokami, Kumamoto, Kumamoto, 860-0862, Japan;3. Mizuho Town Museum, 316-5 Komagata Fujiyama, Mizuho, Nishitama, Tokyo, 190-1202, Japan;4. Faculty of Agriculture, Saga University, 1 Honjo-machi, Saga, Saga, 840-8502, Japan;5. Faculty of Science, Yamagata University, 1-4-12 Kojirakawa, Yamagata, Yamagata, 990-8560, Japan;1. Graduate School of Natural Science and Technology, Kanazawa University, Kakuma-machi, Kanazawa, Ishikawa, 920?1192, Japan;2. Hokkaido University Museum, Hokkaido University, Sapporo, Hokkaido, 060-0810, Japan;1. Universidad Politécnica de Pachuca, Carretera Pachuca-Cd. Sahagún km 20 Ex-Hacienda de Santa Bárbara, Zempoala, Hidalgo, CP 42184, Mexico;2. Laboratorio de Genética Microbiana, Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Prolongación de Carpio y Plan de Ayala s/n, Col. Santo Tomás, Del. Miguel Hidalgo, Ciudad de, C.P. 11340, Mexico;1. Department of Ecology and Protection of Environment, West Pomeranian University of Technology in Szczecin, S?owackiego 17, PL–71434 Szczecin, Poland;2. Department Genetics, Breeding and Biotechnology of Plants, West Pomeranian University of Technology in Szczecin, S?owackiego 17, PL–71434 Szczecin, Poland;1. State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, No. 3 Park 1, Beichen West Road, Chaoyang District, Beijing, 100101, PR China;2. Microbiome Research Center, Moon (Guangzhou) Biotech Ltd., Guangzhou, 510535, PR China |
| |
| Abstract: | Improved understanding of mycorrhizal diversity in mycoheterotrophic (MH) plants is a key element of studies that investigate their evolution. MH plants are completely dependent on their mycorrhizal fungi for carbon. Mycorrhizal fungi of the MH genus Yoania (Orchidaceae), which is distributed in East Asia, have yet to be identified. We identified the mycobionts of three Japanese Yoania species, Y. amagiensis, Y. flava, and Y. japonica, by sequencing the internal transcribed spacer regions of nuclear ribosomal DNA. The sequences obtained were assigned to five operational taxonomic units (OTUs), among which four belonged to the genus Physisporinus (Meripilaceae, Polyporales) and one to Thelephoraceae. Yoania flava and Y. japonica were specifically associated with a single OTU of Physisporinus, while Y. amagiensis was associated with four Physisporinus OTUs. A phylogenetic analysis showed that fungal sequences from species of two other MH orchid genera, Cyrtosia and Galeola, also belonged to Physisporinus and were closely related to the Yoania mycobionts. This is the first study to report that (i) wood-rotting Physisporinus fungi form mycorrhizae with plant species, and (ii) have an important role in orchid mycoheterotrophy. |
| |
| Keywords: | Calypsoeae Mycoheterotrophy Mycorrhizal specificity nrDNA ITS Orchidaceae Wood-decay fungi |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
