Anti-cancer activity of biosynthesized silver nanoparticles using Avicennia marina against A549 lung cancer cells through ROS/mitochondrial damages |
| |
| Institution: | 1. Department of Medical Oncology, 3201 Hospital, No. 783 Tianhan Avenue, Hantai District, Hanzhong, Shaanxi 723000, China;2. Molecular, Cell & Cancer Biology Laboratory, Department of Biochemistry, Bharathidasan University, Tiruchirappalli 620 024, Tamil Nadu, India;3. Department of Pharmacognosy, College of Pharmacy, King Saud University, P.O. BOX 2457, Riyadh 11451, Saudi Arabia;4. Department of Marine Science, Bharathidasan University, Tiruchirappalli 620 024, Tamil Nadu, India |
| |
| Abstract: | The biosynthesized Ag NPs was synthesized by using marine mangrove plant extract Avicennia marina. The synthesized Ag NPs was confirmed by various physiochemical characterization including UV-spectrometer and XRD analysis. In addition, the shape and of the synthesized Ag NPs was morphologically identified by SEM initially and TEM finally. After confirmation, the anti-cancer property of synthesized Ag NPs was confirmed at 50 µg/mL concentration against A549 lung cancer cells by MTT assay. Further, the ability to stimulate the ROS generation and mitochondrial membrane at the IC50 concentration of Ag NPs was confirmed by fluorescence microscopy using DCFH-DA and rhodamine 123 dyes respectively. Finally, the result was concluded that the synthesized Ag NPs has improved anti-cancer activity against A549 cells at lowest concentration. |
| |
| Keywords: | Silver nanoparticle Anti-cancer activity Cytotoxicity assay ROS generation Mitochondrial damage |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
