Preparative HPLC fraction of Hibiscus rosa-sinensis essential oil against biofilm forming Klebsiella pneumoniae |
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| Institution: | 1. Department of Pediatrics, 3201 Hospital, No. 783, Tianhan Avenue, Hantai District, Hanzhong, Shaanxi 723000, China;2. Medical Microbiology and Marine Pharmacology Laboratory, Department of Marine Science, Bharathidasan University, Tiruchirappalli, Tamil Nadu 620024, India;3. Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia;4. Ministry of Education Key Laboratory of Water and Sediment Science, School of Environment, Beijing Normal University, Beijing 100875, China |
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| Abstract: | Recent years Klebsiella pneumoniae (K. pneumoniae) biofilm formation (BF) is emerging thread worldwide. For tackling this problem, we have chosen Hibiscus rosa-. pneumoniae. The HPLC purified essential oils (EOs sinensis (H. rosa-sinensis) (HRS) to inhibit the BF K) of H. rosa-sinensis was performed against BF K. pneumoniae and showed concentration dependent biofilm inhibition. At the MBIC of EOs (90 µg/ml), the biofilm inhibition was showed at 92% against selected BF K. Pneumoniae. The biofilm metabolic assay, exopolysaccharide quantification and hydrophobicity index variation results exhibited with 88%, 92% and 89% reduction at 90 μg/mL was observed respectively. In addition, the morphological modification of MBIC treated K. pneumoniae was clearly viewed by scanning electron microscope (SEM). Overall, all the invitro experiments result were confirmed that the MBIC of H. rosa-sinensis EOs was very effective against BF K. pneumonia. |
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| Keywords: | Biofilm bacteria Medicinal plant Essential oil Anti-biofilm activity Exopolysaccharide Scanning electron microscope |
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