Influence of pH on in vitro mycelial growth in three Japanese truffle species: Tuber japonicum,T. himalayense,and T. longispinosum |
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| Affiliation: | 1. Forestry and Forest Products Research Institute, 1 Matsunosato, Tsukuba, Ibaraki, 305-8687, Japan;2. Kyushu Research Center, Forestry and Forest Products Research Institute, 4-11-16 Kurokami, Chuo, Kumamoto, Kumamoto, 860-0862, Japan;3. Tohoku Research Center, Forestry and Forest Products Research Institute, 92-25 Nabeyashiki, Shimokuriyagawa, Morioka, Iwate, 020-0123, Japan;1. Fungus/Mushroom Resource and Research Center, Faculty of Agriculture, Tottori University, 4-101, Koyama, Tottori, 680-8553, Japan;2. Graduate School of Sustainable Science, Tottori University, 4-101, Koyama, Tottori, 680-8553, Japan;3. The United Graduate School of Agricultural Science, Tottori University, 4-101, Koyama, Tottori, 680-8553, Japan;1. Biological Resource Center (NBRC), National Institute of Technology and Evaluation, 2-5-8 Kazusakamatari, Kisarazu, Chiba, 292-0818, Japan;2. Bioresources Technology Unit, National Center for Genetic Engineering and Biotechnology (BIOTEC), 113 Thailand Science Park, Paholyothin Road, Khlong 1, Khlong Luang, Pathum Thani, 12120, Thailand;3. Herbarium, Biodiversity Unit, University of Turku, FI-20014, Turku, Finland;4. Faculty of Life and Environmental Sciences, University of Tsukuba, 1-1-1, Tennodai, Tsukuba, Ibaraki, 305-8572, Japan;5. Department of Botany, National Museum of Nature and Science, 4-1-1, Amakubo, Tsukuba, Ibaraki, 305-0005, Japan;6. Fungus/Mushroom Resource and Research Center, Tottori University, 4-101 Koyama-Minami, Tottori, Tottori, 680-8553, Japan;1. CONACyT-Departamento de Botánica y Zoología, Universidad de Guadalajara, Apdo. Postal 1–139, Zapopan, Jalisco, 45101, Mexico;2. Coatepec, 91500, Veracruz, Mexico;3. Institute of Geology, Czech Academy of Sciences, Rozvojová 269, 165 00, Prague 6, Czech Republic;4. Departamento de Biología Celular y Molecular, Universidad de Guadalajara, Zapopan, Jalisco, 45202, Mexico;5. Department of Ecology and Evolutionary Biology, University of Tennessee, 332 Hesler Biology Building, Knoxville, TN, 37996–1610, USA;1. Graduate School of Agriculture, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya, Tokyo, 156-8502, Japan;2. Faculty of Regional Environment Science, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya, Tokyo, 156-8502, Japan;1. Universidad Politécnica de Pachuca, Carretera Pachuca-Cd. Sahagún km 20 Ex-Hacienda de Santa Bárbara, Zempoala, Hidalgo, CP 42184, Mexico;2. Laboratorio de Genética Microbiana, Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Prolongación de Carpio y Plan de Ayala s/n, Col. Santo Tomás, Del. Miguel Hidalgo, Ciudad de, C.P. 11340, Mexico;1. Laboratory of Environmental Mycoscience, Graduate School of Agriculture, Kyoto University, Kyoto, 606-8502, Japan;2. Environmental Science Graduate School, The University of Shiga Prefecture, 2500 Hassaka-cho, Hikone City, Shiga, 522-8533, Japan |
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| Abstract: | Truffle species indigenous to Japan include a white-colored truffle Tuber japonicum and two black-colored truffles, T. himalayense and T. longispinosum. As the fruiting bodies of these Tuber species are promising edible, studies on the artificial cultivation for these truffles are currently underway in Japan. In the present study, we investigated the influence of pH on in vitro mycelial growth in these Tuber species to determine the optimal pH conditions for the cultivation of these truffles. Mycelia of five strains from each species were cultured in modified Melin–Norkrans liquid medium at different pH values. Tuber japonicum grew well at pH 5.0 and 6.0, whereas T. himalayense and T. longispinosum grew well at pH 7.0. This results suggest that the optimal pH for mycelial growth varies among Tuber species. The growth data collected in this study can be used to design optimal pH conditions for artificial cultivation of these Japanese truffles. |
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| Keywords: | Ectomycorrhizal fungi Interspecific variation Physiology Truffle cultivation Tuberaceae |
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