Effects of bacterial strains isolated from the ectomycorrhizal roots of Laccaria parva on sporocarp production by the fungus in vitro |
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| Institution: | 1. Tochigi Prefectural Museum, 2-2 Mutsumi-cho, Utsunomiya, Tochigi, 320-0865, Japan;2. Hokken Co. Ltd., 7-3 Ekihigashimachi, Mibu, Shimotsuga-gun, Tochigi, 321-0222, Japan;3. Kanagawa Prefectural Museum of Natural History, 499 Iryuda, Odawara, Kanagawa, 250-0031, Japan;1. Laboratory of Plant Pathology, Institute of Plant Pathology, Jilin Agricultural University, Changchun 13, 130118, Jilin Province, PR China;2. Martin-Luther-Universität, Institut für Biologie, Bereich Geobotanik und Botanischer Garten, Neuwerk 21, 06099, Halle, Saale, Germany;1. College of Food Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China;2. College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China;3. Key Laboratory of Agro-Microbial Resources and Utilization, Ministry of Agriculture, Wuhan, 430070, China;1. Laboratory of Biological Invasion and Ecosecurity, Yunnan University, Kunming, 650091, China;2. China Eco-development Academy, Southwest Forestry University, Kunming, 650224, China;3. FuturaGene Biotechnology (Shanghai) Co., Ltd., Shanghai, 200233, China;4. Yunnan Academy of Forestry, Kunming, 650201, China;1. Microbiome Research Center, Moon (Guangzhou) Biotech Ltd., Guangzhou, 510535, PR China;2. China General Microbiological Culture Collection Centre, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, PR China;1. Forestry and Forest Products Research Institute, 1 Matsunosato, Tsukuba, Ibaraki, 305-8687, Japan;2. Kyushu Research Center, Forestry and Forest Products Research Institute, 4-11-16 Kurokami, Chuo, Kumamoto, Kumamoto, 860-0862, Japan;3. Tohoku Research Center, Forestry and Forest Products Research Institute, 92-25 Nabeyashiki, Shimokuriyagawa, Morioka, Iwate, 020-0123, Japan |
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| Abstract: | The effects of bacteria isolated from ectomycorrhizas on sporocarp production of Laccaria parva were examined in vitro. Three bacterial strains of Bradyrhizobium were selected on the basis of their affinity for L. parva LL02 in previous confrontation tests: a positive or a negative strain that significantly increased or decreased hyphal extension areas, and a neutral strain that did not have significant effects on them. Mycelia of LL02 and a suspension of each bacterial strain were inoculated onto a surface-sterilized pine seedling in a glass bottle and then incubated for 3 mo in an illuminated incubator. Plant biomass did not differ significantly among the treatments, but the level of ectomycorrhizal colonization was low in the treatment inoculated with a negative strain (Neg treatment). The frequency with which mature sporocarps occurred was higher in treatments inoculated with positive (Pos treatment) or neutral strains (Neu treatment) compared with control and Neg treatments. The ratio of biomass accounted for by mature sporocarps was low in control and Neg treatments, and high in Pos and Neu treatments. These results indicate that bacteria colonizing ectomycorrhizas affect the production of sporocarps and that the affinity between fungi and bacteria is likely to underlie this interaction. |
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| Keywords: | Fruit body Hyphal extension Interaction Maturation Mycorrhizal colonization |
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