Chromosome analysis and FISH mapping of ribosomal DNA (rDNA), telomeric (TTAGGG)n and (GATA)n repeats in the leech Haemopis sanguisuga (L.) (Annelida: Hirudinea) |
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Authors: | Vitturi R Libertini A Armetta F Sparacino L Colomba M S |
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Institution: | (1) Dipartimento di Biologia Animale, Università di Palermo, Via Archirafi 18, 90123 Palermo, Italy (Phone;(2) CNR, Istituto di Biologia del Mare, Riva 7 Martiri 1364/A, 30122 Venezia, Italy;(3) Facoltà di Scienze Ambientali, Università di Urbino, Località Crocicchia, 61029 Urbino (PU), Italy |
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Abstract: | In the present paper the chromosome complement (n = 13; 2n = 26) of the common leech Haemopis sanguisuga (L.) (Annelida: Hirudinea: Hirudinidae) was analyzed using banding techniques and fluorescent in situ hybridization (FISH) with three repetitive DNA probes ribosomal DNA (rDNA), (TTAGGG)
n
and (GATA)
n
]. FISH with the rDNA probe consistently mapped major ribosomal clusters (18S–28S rDNA) in the pericentromeric region of one large metacentric chromosome pair; this region, which consisted of heterochromatin rich in GC base pairs, was preferentially stained by silver nitrate (Ag-NOR). The (TTAGGG)
n
telomeric probe was hybridized with the termini of nearly all chromosomes, whereas the (GATA)
n
probe did not label any chromosome areas. |
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Keywords: | chromosomes FISH Haemopis sanguisuga Hirudinea repetitive DNA |
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