云南南部野生大叶千斤拔资源遗传多样性的ISSR分析

应用ISSR分子标记技术,对云南南部7个地区的野生大叶千斤拔( Flemingia macrophylla)居群进行了遗传多样性分析。结果表明：云南野生大叶千斤拔具有较高的遗传多样性。在物种水平上,平均每个位点的多态位点百分率(PPL)为94.85％,有效等位基因数(Ne)为1.4627,Nei’s基因多样性指数(He)为0.2815, Shannon’s多样性信息指数(Ho)为0.4337；在居群水平上,PPL ＝43.44％,Ne ＝1.2981,He ＝0.1704,Ho ＝0.2499。基于Nei’ s遗传多样性分析可得出,居群间的遗传分化系数( Gst)为0.3975,表明居群内的遗传变异为60.25％,居群间的遗传变异为39.75％,这说明居群间的遗传分化要低于居群内的遗传分化。根据遗传多样性分析和聚类结果,应在大叶千金拔遗传多样性较高的勐腊易武( MY)、丘北( QB)和宁洱( NE)地区,设立保护点对其进行就地保护。

Analysis of genetic diversity of Flemingia macrophylla resources from South Yunnan by ISSR

The genetic diversity of 135 individuals collected from 7 wild populations of Flemingia macrophylla was estimated using ISSR method. An high level of genetic diversity was detected in wild populations of F. macrophylla from South Yunnan Province. At species level, the percentage of polymorphic loci PPL = 94.85%, effective number of alleles Ne = 1.462 7, Nei''s gene diversity He = 0.281 5, and Shannon''s information index Ho = 0.433 7; at population level, PPL = 43.44%, Ne = 1.298 1, Nei''s gene diversity He = 0.170 4, and Shannon''s in formation index Ho = 0.249 9. The level of genetic differentiation(39.75%)among populations is lower than that within populations(60.25%). Based on the results, we suggest that the protected areas of F. macrophylla should be build in MY, QB and NE because of the high level of genetic diversity.