Overexpression of wheat mitochondrial uncoupling protein in rice plants confers tolerances to oxidative stresses promoted by exogenous hydrogen peroxide and low temperature |
| |
| Authors: | Kenjirou Ozawa Seiji Murayama Ai Kobayashi-Uehara Hirokazu Handa |
| |
| Affiliation: | (1) Department of Low-Temperature Sciences, National Agricultural Research Center for Hokkaido Region, 1␣Hitsuji-ga-oka, Toyohira-ku, Sapporo 062-8555, Japan;(2) Department of Plant Biotechnology, National Institute of Agrobiological Sciences, 2-1-2 Kan-non-dai, Tsukuba 305-8602, Japan;(3) Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Ten-noh-dai, Tsukuba 305-8572, Japan |
| |
| Abstract: | To obtain a better understanding of the function of mitochondrial uncoupling protein (UCP) in higher plants, the wheat gene for mitochondrial uncoupling protein (WhUCP) in rice was overexpressed by Agrobacterium-mediated transformation with a construct containing the WhUCP ORF under control of the 35S promoter. The transgenic rice plants showed a significant increase in tolerance against oxidative stress promoted by exogenous hydrogen peroxide at the seedling stage. The transgenic rice plants overexpressing WhUCP also exhibited greater tolerance against cold stress than did the wild-type plants. These results demonstrated that the mitochondrial UCP in higher plants is positively involved in the pathway for abiotic stress tolerance, probably through a decrease in cellular oxidative damage, and that controlled uncoupling by UCP could be used for improvement of stress tolerance in higher plants.Kenjirou Ozawa and Seiji Murayama are contributed equally to the work |
| |
| Keywords: | Cold tolerance Mitochondria Oxidative stress Transgenic rice plants UCP (uncoupling protein) Wheat |
| 本文献已被 SpringerLink 等数据库收录！ |
|
