Effects of vibration on differentiation of cultured PC12 cells |
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Authors: | Ito Yukiko Kimura Tsuyoshi Nam Kwangwoo Katoh Ayako Masuzawa Toru Kishida Akio |
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Affiliation: | Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, Tokyo, Japan. |
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Abstract: | Different types of physiological‐mechanical stress, such as shear stress in vascular endothelial cells or hydrostatic pressure in chondrocytes are well known as regulators of cell function. In this study, the effects of vibration, a type of non‐physiological mechanical stimulation, on differentiation of rat pheochromocytoma (PC12) cells are reported. A nano‐vibration system was designed to produce nanometer‐scale vibration. The frequency and amplitude of the nano‐vibrations were monitored by a capacitance displacement sensor connected to an oscilloscope. When PC12 cells exposed to nerve growth factor were subjected to vibration at 10 kHz, differentiation and elongation of their neurites were promoted earlier in the culture. Vibration promoted differentiation of PC12 cells. This approach could therefore also be promising for determining of the effects of the physical environment on cell differentiation. Biotechnol. Bioeng. 2011; 108:592–599. © 2010 Wiley Periodicals, Inc. |
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Keywords: | nano‐vibration PC12 cell morphological analysis differentiation |
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